A laboratory-scale milk pasteurization system using pulsed electric field technique was developed and presented in this paper. This developed system was consists of a DC pulsed high voltage power supply, a treatment chamber and a fluid flow system. Milk was transported from the milk tank into the treatment chamber at a flow rate of 2 L/min. In the chamber, a DC pulsed high voltage was applied to the electrodes of about 30 kV with frequency of about 30 kHz, therefore, the high electric field strength was found 40 kV/cm. In this study, the developed system was preliminary tested with a low-fat and sterilized milk mixed with an E.coli. In the initial infection, it was found from the preliminary test that the E.coli was reduced by about 96.67% of with the residence time of about 30 min. The milk temperature was increased after the chamber of about 2 - 3 ° C from the room temperature. The energy of the system was equivalent to about 0.091 kWh.