The aim of this study was to evaluate the acute effect of energy boosting supplement in athlete and sedentary . The method was a randomized double-blind controlled trial. Subjects were 15 males athletes and 15 sedentary men with an average age of 20.70 ± 1.37 years. Three sessions of study was set up as the 1st (control), 2nd (placebo) and/or 3rd (supplement) sessions. After supplementation males the boosting supplement or placebo for 30 min, energy using capacity as oxygen consumption (VO2), respiratory rate (RR), and minute ventilation (VE) were evaluated with a direct breath – by - breath technique while subjects were running on treadmill at 80% of maximal heart rate (MHR). All parameters from three sessions including data at 80% MHR and mean pre-post different values were analyzed for statistical difference with repeated measurement ANOVA (2 groups x 3 times) with SPSS program significance being set at p ≤ 0.05. Our results showed that supplementation of boosting nutrient (Bios Life E) did not affect the oxygen consumption (VO2), respiratory rate (RR), and minute ventilation (VE) at 80% of MHR and pre-post different values, when compared to the control and placebo periods both in athletics and sedentary subjects. To sum up, our study revealed no evidence of energy boosting effects. Bios Life E supplement in both male athletes and sedentary subjects.