Evaluation of total soluble solids of strawberry fruit cv. No. 80 using near infrared (NIR) spectroscopy with wavelength ranging from 700 nm to 1100 nm was studied. NIRSystem6500 was used to acquire the spectrum while a total soluble solids (TSS) was measured using digital refractometer. The fruit was separated into 2 groups by percentage of red color break using the following criteria: white stage; less than 25% color break and red stage; more than 75% color break. The soluble solids content of white stage equalled to 10.02% and was significantly different from total soluble solids of red stage (10.66%). The partial least squares regression (PLSR) models for prediction TSS of strawberry showed that the calibration equations formulated by the relationship between values of spectrum and TSS could predict TSS values with the correlation coefficient; R equal to 0.81, the standard error of calibration (SEC) and the standard error of prediction (SEP) equal to 0.46 and 0.49, respectively.