| ชื่อเรื่อง | : | Production and characterization of anti-dengue capsid antibodies suggesting the N terminus region covering the first 20 amino acids of dengue virus capsid protein is predominantly immunogenic in mice |
| นักวิจัย | : | Puttikhunt C. , Ong-ajchaowlerd P. , Prommool T. , Sangiambut S. , Netsawang J. , Limjindaporn T. , Malasit P. , Kasinrerk W. |
| คำค้น | : | - |
| หน่วยงาน | : | มหาวิทยาลัยเชียงใหม่ |
| ผู้ร่วมงาน | : | - |
| ปีพิมพ์ | : | 2552 |
| อ้างอิง | : | 03048608 , 10.1007/s00705-009-0426-5 , 19565324 , ARVID , http://www.scopus.com/inward/record.url?eid=2-s2.0-70349560295&partnerID=40&md5=1ef84007d5708763cd2b5feb14cc22fb , http://cmuir.cmu.ac.th/handle/6653943832/770 |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | - |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | We produced monoclonal and polyclonal antibodies to the capsid (C) protein of dengue serotype 2 virus (DV2 C). First, a maltose-binding protein fused to DV2 C protein (MBP-C) was overproduced in E. coli. The affinity-purified MBP-C protein was cleaved by factor Xa protease to obtain a recombinant DV2 C protein, which was then used for mouse immunizations. Two hybridoma cell lines producing anti-C Mabs as well as anti-C polyclonal antibody were successfully generated and characterized. Interestingly, all of the generated antibodies specifically recognized the first 20 amino acids of the DV2 C protein, as determined by peptide epitope mapping and via a recombinant DV2 C protein in which this region was deleted. The results suggested that this region is predominantly immunogenic in mice. © Springer-Verlag 2009. |
| บรรณานุกรม | : |
Puttikhunt C. , Ong-ajchaowlerd P. , Prommool T. , Sangiambut S. , Netsawang J. , Limjindaporn T. , Malasit P. , Kasinrerk W. . (2552). Production and characterization of anti-dengue capsid antibodies suggesting the N terminus region covering the first 20 amino acids of dengue virus capsid protein is predominantly immunogenic in mice.
เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ . Puttikhunt C. , Ong-ajchaowlerd P. , Prommool T. , Sangiambut S. , Netsawang J. , Limjindaporn T. , Malasit P. , Kasinrerk W. . 2552. "Production and characterization of anti-dengue capsid antibodies suggesting the N terminus region covering the first 20 amino acids of dengue virus capsid protein is predominantly immunogenic in mice".
เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ . Puttikhunt C. , Ong-ajchaowlerd P. , Prommool T. , Sangiambut S. , Netsawang J. , Limjindaporn T. , Malasit P. , Kasinrerk W. . "Production and characterization of anti-dengue capsid antibodies suggesting the N terminus region covering the first 20 amino acids of dengue virus capsid protein is predominantly immunogenic in mice."
เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2552. Print. Puttikhunt C. , Ong-ajchaowlerd P. , Prommool T. , Sangiambut S. , Netsawang J. , Limjindaporn T. , Malasit P. , Kasinrerk W. . Production and characterization of anti-dengue capsid antibodies suggesting the N terminus region covering the first 20 amino acids of dengue virus capsid protein is predominantly immunogenic in mice. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2552.
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