Differentiated bone marrow cells from adult rats can differentiate into osteoblast-like cells in vitro and form bone in vivo. Autogenous bone marrow harvesting and implantation and osteogenic cell culture procedures were established. Cell seeding density and immunological response of host cells to substances used in cell culture are critical factors determining bone forming capacity of the implanted osteoblasts. Effects of autogenous growth factors derived from PC can not be demonstrated in this study.

    In further study, to obtain a maximum amount of bone formation density of cell seeding should be at least 2-5x10⁵ cells / 3x5 mm scaffolds. Cells should be cultivated into 3^rd passage and trypsinized for cell seeding at 80% confluence to prevent cell clumping. Seeded cells should be allowed to attachment on the scaffolds in a minimum culture medium for at least two hours before an application of growth factors. Scaffolds and cells should be extensively washed in culture medium without.