The isolations of active anticancer compounds from Solanum spirale Roxb.,
 collected from Phayao Province, Thailand are rarely reported. The
anticancer activities of hexane and methanol extracts of the leaves were
 investigated. The chloroform extract possessed anticancer activities
against KB-Oral cavity cancer, MCF-7 breast cancer cell and
NCI-H187-Small cell lung cancer with the IC₅₀ values of 42.73, 17.90, and 36.74 µg/mL, respectively. But
 the hexane extract did not inhibit significant anticancer activity
against three cancer cell lines. Lupeol, protocatechuic acid and trans-cinnamic
 acid have been isolated from the chloroform extract of this medicinal
plants. Their structures were characterized on the basis of
spectroscopic analysis. The anticancer activities of the isolated
compounds were also evaluated in vitro against
 three cancer cell lines. Only lupeol compound inhibited significant
anticancer activity against KB-Oral cavity cancer with the IC_50­ value of 26.73 µg/mL, whereas protocatechuic acid and trans-cinnamic
 acid did not exhibit anticancer activity. The hexane, chloroform
extracts and the isolated compounds did not show cytotoxic activity
against the African green monkey kidney cell line. The hexane and
chloroform extracts also inhibited antibacterial activity against Escherichia coli and Staphylococcus aureus with the MIC values were in the range between 375-1500 µg/mL. All isolated compounds showed equal antibacterial activity against E. coli with the MIC of  250 mg/mL, and trans-cinnamic acid showed better antibacterial activity against S. aureus with
 the MIC of  250 mg/mL than lupeol and protocatechuic acid. This is the
first report decribes the isolation and structure elucidation of lupeol,
 protocatechuic acid and trans-cinnamic acid  together with their anticancer, cytotoxic and antibacterial activities from this medicinal plant.