Objective: To develop a simple questionnaire to use for screening Thai people for knee osteoarthritis (OA). Material and Method: Subjects' characteristics and data covering all known risk factors for OA were collected from a sample of villagers at Kanleurng Sub-District, Nakronpanom Province. Hip, knee, and finger radiographs were taken from all subjects (n = 146) to diagnose OA. Multiple logistic regression was used to select the items contained in the questionnaire. A ROC curve was used to define the best cut-off point and distinguish between OA and non-OA. Results: Three factors were identified as being associated with knee OA and were included in a questionnaire. The Thai-KOASQ included questions about knee pain, age and body mass index (BMI). The questionnaire has 79.2% sensitivity, 78.4% specificity and 85.1% for the area under the ROC curve. Conclusion: This questionnaire should be validated in a large group of people, in order to be further developed as the screening questionnaire for knee OA in the general Thai population.