Seed morphometric characters and oil content were studied in multiple-use plant, Calophyllum inophyllum L. of two countries, Australia (southern hemisphere) and Sri Lanka (northern hemisphere). Seven provenances were selected which included three from northern Australia and four from Sri Lanka. Twelve Candidate plus trees (CPTs) each were selected from 2 to 3 different locations within each provenance based on the morphometric and qualitative traits (GBH > 100 cm). Seed collection in both hemispheres was carried out from May to August 2008. Calophyllum inophyllum L. provenances showed a distinct hemisphere variation in their seed-related characters and oil content. All provenances differed significantly (P < 0.05) with one another in seed length. Seeds from Anuradhapura, Sri Lanka and seeds from Cardwell Australia recorded the highest (≈57%) and the lowest oil (≈31%) content respectively. Strong correlations were found between seed morphometric characters. Relatively weaker correlations were found between seed morphometric characters and oil content. Variations in seed-related characters were largely attributed by the provenance contribution which shows the significance of the effect of genetic variability on above mentioned seed related characters.