| ชื่อเรื่อง | : | Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes |
| นักวิจัย | : | De Hoog, Hans-Peter M. , Lin JieRong, Esther M. , Banerjee, Sourabh , Décaillot, Fabien M. , Nallani, Madhavan |
| คำค้น | : | DRNTU::Engineering::Materials::Biomaterials |
| หน่วยงาน | : | Nanyang Technological University, Singapore |
| ผู้ร่วมงาน | : | Karnik, Sadashiva |
| ปีพิมพ์ | : | 2557 |
| อ้างอิง | : | De Hoog, H.-P. M., Lin JieRong, E. M., Banerjee, S., Décaillot, F. M., & Nallani, M. (2014). Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. PLoS One, 9(10), e110847-. , 1932-6203 , http://hdl.handle.net/10220/24190 , http://dx.doi.org/10.1371/journal.pone.0110847 |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | PLoS One |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | G-protein coupled receptors (GPCRs) play a key role in physiological processes and are attractive drug targets. Their biophysical characterization is, however, highly challenging because of their innate instability outside a stabilizing membrane and the difficulty of finding a suitable expression system. We here show the cell-free expression of a GPCR, CXCR4, and its direct embedding in diblock copolymer membranes. The polymer-stabilized CXCR4 is readily immobilized onto biosensor chips for label-free binding analysis. Kinetic characterization using a conformationally sensitive antibody shows the receptor to exist in the correctly folded conformation, showing binding behaviour that is commensurate with heterologously expressed CXCR4. |
| บรรณานุกรม | : |
De Hoog, Hans-Peter M. , Lin JieRong, Esther M. , Banerjee, Sourabh , Décaillot, Fabien M. , Nallani, Madhavan . (2557). Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes.
กรุงเทพมหานคร : Nanyang Technological University, Singapore. De Hoog, Hans-Peter M. , Lin JieRong, Esther M. , Banerjee, Sourabh , Décaillot, Fabien M. , Nallani, Madhavan . 2557. "Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes".
กรุงเทพมหานคร : Nanyang Technological University, Singapore. De Hoog, Hans-Peter M. , Lin JieRong, Esther M. , Banerjee, Sourabh , Décaillot, Fabien M. , Nallani, Madhavan . "Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes."
กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print. De Hoog, Hans-Peter M. , Lin JieRong, Esther M. , Banerjee, Sourabh , Décaillot, Fabien M. , Nallani, Madhavan . Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.
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