Thesis (M.Sc. in Pharm)--Chulalongkorn University, 2003

To investigate effects of n-heptanoyl, nitro-m-methoxy anilide derivatives (CU 18-14 and CU 18-15) on isolated rat liver mitochondrial functions. Effects of CU 18-14 and CU 18-15 on mitochondrial respiration with glutamate plus malate as substrates were as following: did not show any effect on state 3 and state 4 of mitochondrial respiration at low concentration (1.01 x 10 [subscript -6] M), showed the stimulating effect on state 4 of mitochondrial respiration at higher concentration (at 1.01 x 10 [subscript -5] M and higher) and inhibited both state 3 and state 4 of mitochondrial respiration at concentration 3.04 x 10 [subscript -4] M. The similar results was shown on mitochondrial respiration was also found using succinate was used as a substrate. These results suggested that CU 18-14 and CU 18-15 acted as an uncoupler of oxidative phosphorylation. Calcium- and DNP- stimulated respiration were inhibited by these derivatives. CU 18-14 has stronger stimulating effect on ATPase activity than CU 18-15. Furthermore, CU 18-14 and CU 18-15 inhibited both MAO-A and MAO-B activities. CU 18-14 demonstrated a more inhibitory potency for MAO-A activity than CU 18-15