- Jujube is a local fruit widely found in Thailand. Recently new varieties of jujube have been produced from the cross breeding. Various parts of jujube possess biological activities with supportive scientific evidences. However, there is no report of biological activity of the new cross bred jujube. Therefore, this study was conducted to determine the potential of 6 jujube varieties collected from Northeast Thailand on health benefit. The test jujubes were Samros, Rianthong, Bombay, Apple, Taiwan, and Nomsod. The antiglycation, anticancer and immunomodulation effect of the test jujubes were investigated in vitro. Our results showed different antiglycation, anticancer and immunomodulation activities among jujube varieties. The 50% ethanolic extract of raw jujubes contained higher phenolics contents, exhibited greater %inhibition of DPPH and reducing ability based on FRAP assay than the ripen ones. Antiglycation effects of the test jujubes were ranged 51-71% depending on their varieties and ages. The raw jujubes demonstrated higher antiglycation than the ripen ones. Samros has the highest antiglycation in contrast to Rianthong. The cytotoxicity of water extract of various jujubes against the human cancer cell lines at 24 hours exposure was evaluated in comparison to the normal cell lines (i.e., Vero African green monkey kidney cells) and peripheral blood mononuclear cells or PBMC). The cancer cell lines studied were human cancer cells from cervical (SiHa), colon (HCT-116), liver (HepG2), breast (MCF-7), and blood cells (Jurkat and U937). Results showed that Bombay and Taiwan were toxic against the leukemic Jurkat cell line with moderate and low toxicity, respectively. Bombay and Taiwan induced relative low apoptosis cancer dead mode <13%. The water extracts of all jujube varieties at the concentration of 50, 250, and 1000 μg/ml likely to increase immune cell proliferation but not significantly difference, increased pro-inflammatory cytokine IL-1β, cytokine IL-10 from Th-2. Bombay jujube showed most effect on the immune system because it not only induced the highest secretion of IL-1β and IL-10 compared to the other 5 jujube varieties but it also stimulated the secretion of TNF-α (Th-1/NK cell). The water extracts of all jujubes tested reduced cellular stress from the decrease of hydrogen peroxide. In summary, Bombay jujube variety gained the highest interest because it mostly affected the immunomodulating. Further investigation to describe the effect on the immune system should be carried on.