| ชื่อเรื่อง | : | Production Of Novel Recombinant Antipfhrp2 Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System |
| นักวิจัย | : | Kok, Boon Hui |
| คำค้น | : | T1-995 Technology(General) |
| หน่วยงาน | : | Universiti Sains Malaysia, Malaysia |
| ผู้ร่วมงาน | : | - |
| ปีพิมพ์ | : | 2563 |
| อ้างอิง | : | http://eprints.usm.my/49503/1/Kok%20Boon%20Hui.pdf%20cut.pdf , Kok, Boon Hui (2020) Production Of Novel Recombinant Antipfhrp2 Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System. Other. Universiti Sains Malaysia. (Submitted) |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | http://eprints.usm.my/49503/ |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | Malaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are widely used in RDTs but it can be easily degraded at high ambient temperatures. Hence, the shark VNARS might be good alternatives to mAbs due to its higher thermal stability and binding affinity with antigens. In this study, the recombinant anti- PfHRP2 VNAR-G1 protein was produced in E. coli expression system through various steps such as recombinant cell isolation, PCR, agarose gel electrophoresis, plasmid extraction, transformation and protein expression. Besides, the combinatorial effects of temperature and IPTG concentration towards the cell density of recombinant BL21(DE3) based on the absorbance readings and cell wet weights were investigated using software R. |
| บรรณานุกรม | : |
Kok, Boon Hui . (2563). Production Of Novel Recombinant Antipfhrp2
Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System.
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia. Kok, Boon Hui . 2563. "Production Of Novel Recombinant Antipfhrp2
Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System".
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia. Kok, Boon Hui . "Production Of Novel Recombinant Antipfhrp2
Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System."
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia, 2563. Print. Kok, Boon Hui . Production Of Novel Recombinant Antipfhrp2
Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System. กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia; 2563.
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