| ชื่อเรื่อง | : | The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28 |
| นักวิจัย | : | Poobathy, Ranjetta |
| คำค้น | : | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| หน่วยงาน | : | Universiti Sains Malaysia, Malaysia |
| ผู้ร่วมงาน | : | - |
| ปีพิมพ์ | : | 2555 |
| อ้างอิง | : | http://eprints.usm.my/42305/1/RANJETTA_POOBATHY.pdf , Poobathy, Ranjetta (2012) The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28. Masters thesis, Universiti Sains Malaysia. |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | http://ezproxy.usm.my/login?url=http ://ethesis.usm.my/jspui , http://eprints.usm.my/42305/ |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | The vitrification and encapsulation-dehydration methods of cryopreservation were applied on protocorm-like bodies (PLBs) of the orchid hybrid Dendrobium sonia-28, with survival assessments conducted through growth observations, visualand spectrophotometric 2,3,5-triphenyltetrazolium chloride (TTC) assays. The best survival (16.0% regeneration) for vitrification was obtained when 3-4mm PLBs were precultured in semi-solid half-strength MS medium supplemented with 0.4M sucrose, loaded in a loading solution for 20 minutes, dehydrated for 50 minutes at 0°C in plant vitrification solution 2 (PVS2), cryopreserved in liquid nitrogen (LN) for 24 hours, thawed in a 40±2°C water bath for 90 seconds, unloaded in 1.2M sucrose for 20 minutes, and regenerated in semi-solid half-strength MS medium containing 2g.L-1 charcoal, with all media supplemented with 0.6mM ascorbic acid. |
| บรรณานุกรม | : |
Poobathy, Ranjetta . (2555). The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28.
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia. Poobathy, Ranjetta . 2555. "The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28".
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia. Poobathy, Ranjetta . "The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28."
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia, 2555. Print. Poobathy, Ranjetta . The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28. กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia; 2555.
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