| ชื่อเรื่อง | : | Screening and characterization of metagenome-derived PHA synthase gene (phaC) from limestone soil sample of Gunung Lang, Ipoh, Perak. |
| นักวิจัย | : | Kanapathi Pillai, K. Sudesh Kumar |
| คำค้น | : | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| หน่วยงาน | : | Universiti Sains Malaysia, Malaysia |
| ผู้ร่วมงาน | : | - |
| ปีพิมพ์ | : | - |
| อ้างอิง | : | Kanapathi Pillai, K. Sudesh Kumar Screening and characterization of metagenome-derived PHA synthase gene (phaC) from limestone soil sample of Gunung Lang, Ipoh, Perak. Project Report. Universiti Sains Malaysia. (Submitted) |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | http://eprints.usm.my/32358/ |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | In this study, metagenomic approach was applied to identify novel PHA synthase genes (phaCs). The presence of phaCs in limestone soil was screened. Phylogenetic analysis has revealed that limestone area harbors a great diversity of phaCs. A total of 87 partial phaCs were obtained. Among these, 7 partial phaC genes were selected for full length amplification through metagenome walking. One of the clones (SG8) encoded a new gene (PhaCsca) , with an 81 % identity to the phaC of Chromobacterium vio/aceum ATCC 12472 based on BLASTn results. It able to express successfully and produce up to 55 wt. % of PHA when it was cloned into a PHA-negative mutant of Cupriavidus necator PHS-4. Besides that, the PHA synthase activity of this transformant was 55 U/g protein. |
| บรรณานุกรม | : |
Kanapathi Pillai, K. Sudesh Kumar . (). Screening and characterization of metagenome-derived PHA synthase gene (phaC) from limestone soil sample of Gunung Lang, Ipoh, Perak..
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia. Kanapathi Pillai, K. Sudesh Kumar . . "Screening and characterization of metagenome-derived PHA synthase gene (phaC) from limestone soil sample of Gunung Lang, Ipoh, Perak.".
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia. Kanapathi Pillai, K. Sudesh Kumar . "Screening and characterization of metagenome-derived PHA synthase gene (phaC) from limestone soil sample of Gunung Lang, Ipoh, Perak.."
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia, . Print. Kanapathi Pillai, K. Sudesh Kumar . Screening and characterization of metagenome-derived PHA synthase gene (phaC) from limestone soil sample of Gunung Lang, Ipoh, Perak.. กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia; .
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