The Addenbrooke’s Cognitive Examination Revised (ACE-R) is a brief and highly sensitive screening instrument designed to detect a cognitive impairment in people with dementia. Its accuracy and usefulness in the diagnosis is reported by a number of validation studies worldwide. However, the test is not used a lot in the post-communist bloc. Therefore the main aim of the current study was to translate and adapt the ACE-R into Slovak, evaluate its psychometric properties in the elderly population of the country and check its generalizability and cross-cultural adaptability. It was also attempted to compare its usefulness and accuracy with the MMSE and show its advantage over this dementia screening tool widely used in Slovakia. A patient group, composed of 31 people with dementia, was compared with 52 healthy individuals matched for age, gender and educational level. Standard statistical tests were applied in order to evaluate the psychometric properties of the Slovak ACE-R adaptation. Education and age were shown to have an effect on the test performance. Reliability of the test was excellent (Cronbach’s alpha= 0.9), so was the diagnostic accuracy (AUC value of 0.989). The accuracy and partial cut-offs of all subtests were also calculated using the ROC curve with obtaining an excellent or good value of the AUC. Two cut-off scores were defined, 80 for MCI (sensitivity= 90%, specificity= 100%) and 77 for dementia (94%, 90%). Furthermore, the performance of the patient group and also the participants with a borderline score was analysed in detail. The Slovak ACE-R was proven to be a practical and sensitive screening tool for identification of a cognitive impairment in people with dementia in Slovakia.